Products Description
The Singclean afp tumor marker test was developed based on an enzyme-linked immunosorbent assay (ELISA). It diagnoses the presence of primary hepatocellular carcinoma or open fetal neural tube defects by detecting fetal proteins in humans, as AFP will present at a higher than normal level in the patient and therefore can determine if the disease is present. However, the result can only be used as a primary test, and the diagnosis of liver cancer should be made by However, the result can only be used as a primary test, and the diagnosis of liver cancer should be made by further testing.
characteristic
Detection limit -Detection limit ≤5.0 ng/mL;
Linearity-AFP concentration- OD value curve correlation coefficient (T) 0.9900 in the detection range;
Accuracy-The relative deviation of detected concentration to target concentration ≤ 15.0%;
Precision-coefficient of variation (CV)≤15.0% in the detection range;
Specificity-No less than 10 serum samples, all the detected concentration is <20.0ng.
specification
48 tests / kit, 96 tests / kit
Equipment required but not supplied with the kit
1. ELISA analyzer (with a wavelength of 450 nm , 630 nm).
2. Precision pipettes (pipettes with disposable plastic tips for delivery of 50,100μL is applicable, and it is alternative for other applicable automatic sampler).
3. Thermotank (37℃incubator, alternative for other thermostatic equipment).
4. Wash device (automatic washing machine is recommended).
5. Microplate shaker.
test procedure
Allow all reagents to reach room temperature before use:
1. Dilute wash buffer (20×) (1:19) with distilled water.
2. Label or mark the micro well strips to be used on the plate.
3. Pipet 50μL of AFP standards (0,5,20,50,200,500 ng/mL),AFP diluent (users as supplied), and serum samples into appropriate wells, mixed well, cover the plate and incubated at 37°C for 30min.
4. Aspirate and wash the wells 3 times with wash buffer. We recommend using an automatic ELISA plate washer for better consistency.
5. Add 100uL of anti-AFP-HRP conjugate reagent into each well. Cover the plate and incubate at 37°C for 30 min.
6. Aspirate and wash the wells 3 times with wash buffer as above.
7. Dispense 100μL HRP substrate solution into each well. Mix gently,cover the plate and incubate for 10 min at 37℃.
8. Add 50μL stop solution into each well and mixed gently.
9. Read optical density (OD) value at 450 nm in Elisa Analyzer within 30 minutes after addition of the stop solution.
10. The standard curve and regression equation were established by AFP standards color intensity,then calculate the AFP concentration in samples (Correlation coefficient(r)≥0.9900).
limitations and Warning
It is not applicable for the whole blood samples detection, serving as assistant in vitro diagnosis, the result should not be as absolute evidence.
FAQ
Q1:Can I have a sample for our testing?
A1: Yes, stock samples are available and but you need to pay the freight.
Q2:What is sample ready time?
A2: Usually 3-5 days, since it was produced against specific demands to guarantee most freshness.
Q3:What about the payment methods?
Paypal, Western Union, T/T Q4 :What's your delivery time?
A4: Usually 5-35 days after receiving the deposit.
Q5:What's your MOQ of each product?
A5: Lowest MOQ but price is different for different quantity. If you have large quantity, please kindly contact us for charming price.
Q6: How to deal with the products regarding the quality and quantity?
A6: Singclean medical is a professional supplier for the medical products for many years. it is of great importance for the quality issues, QC department will arrange the checking every lot, also we have the spot checking at random, we keep high concentration of quality issues, also quantity issues , which is the life of a company, we already got CE, ISO, SGS and FSC certification.
Q8:Can i have a phone call directly with your sales if some urgent questions?
A8: yes, of course, that is wonderful to save the time and catch the key point easily. our sales team members can speak fluent SPANISH, RUSSIAN, GERMAN, JAPANESE, ENGLISH. If you have any questions, you can call us or send us by email.
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